Bennett, Lori, Yang, Ming, Enikolopov, G. N., Iacovitti, Lorraine (June 2009) Circumventricular organs: A novel site of neural stem cells in the adult brain. Molecular and Cellular Neuroscience, 41 (3). pp. 337-347.
Abstract
Neurogenesis in the adult mammalian nervous system is now well established in the subventricular zone of the anterolateral ventricle and subgranular zone of the hippocampus. In these regions, neurons are thought to arise from neural stem cells, identified by their expression of specific intermediate filament proteins (nestin, vimentin, GFAP) and transcription factors (Sox2). In the present study, we show that in adult rat and mouse, the circumventricular organs (CVOs) are rich in nestin+, GFAP+, vimentin+ cells which express Sox2 and the cell cycle-regulating protein Ki67. In culture, these cells proliferate as neurospheres and express neuronal (doublecortin+, [beta]-tubulin III+) and glial (S100[beta]+, GFAP+, RIP+) phenotypic traits. Further, our in vivo studies using bromodeoxyuridine show that CVO cells proliferate and undergo constitutive neurogenesis and gliogenesis. These findings suggest that CVOs may constitute a heretofore unknown source of stem/progenitor cells, capable of giving rise to new neurons and/or glia in the adult brain.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Neural stem cell Neurogenesis Gliogenesis Nestin Sox2 Ki67 Vimentin |
| Subjects: | organism description > animal > developmental stage > adult organs, tissues, organelles, cell types and functions > organs types and functions > brain organs, tissues, organelles, cell types and functions > organs types and functions organs, tissues, organelles, cell types and functions therapies > stem cells |
| CSHL Authors: | |
| Communities: | CSHL labs > Enikopolov lab CSHL Cancer Center Shared Resources > Animal Services CSHL Cancer Center Shared Resources > Microscopy Service |
| Depositing User: | Matt Covey |
| Date: | 22 June 2009 |
| Date Deposited: | 21 Feb 2013 19:31 |
| Last Modified: | 30 Dec 2014 16:58 |
| PMCID: | PMC2697272 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27343 |
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