Trans splicing of nematode pre-messenger RNA in vitro

Hannon, G. J., Maroney, P. A., Denker, J. A., Nilsen, T. W. (1990) Trans splicing of nematode pre-messenger RNA in vitro. Cell, 61 (7). pp. 1247-1255. ISSN 00928674 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2163760
DOI: 10.1016/0092-8674(90)90689-c

Abstract

In nematodes, a fraction of mRNAs contains a common 22 nucleotide 5' terminal spliced leader (SL) sequence derived by trans splicing. Here, we show that a cell-free extract prepared from developing embryos of the parasitic nematode Ascaris lumbricoides catalyzes accurate and efficient SL addition to a synthetic pre-mRNA at an authentic trans splice acceptor site. SL addition occurs via a trans splicing reaction that proceeds through Y-branched intermediates. The branch-point is located at either of two adenosine residues located 18 and 19 nucleotides upstream of the splice acceptor site.

Item Type: Paper
Uncontrolled Keywords: messenger rna precursor article ascaris nematode nonhuman priority journal rna splicing trans splicing Animal Base Sequence Endoribonucleases Molecular Sequence Data Nucleotide Mapping Oligonucleotide Probes Ribonuclease H, Calf Thymus Ribonuclease T1 RNA Precursors RNA, Messenger Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Ascaris lumbricoides Nematoda
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 1990
Date Deposited: 09 Jan 2013 17:35
Last Modified: 09 Jan 2013 17:35
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26502

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