U small nuclear ribonucleoprotein requirements for nematode cis- and trans-splicing in vitro

Hannon, G. J., Maroney, P. A., Nilsen, T. W. (1991) U small nuclear ribonucleoprotein requirements for nematode cis- and trans-splicing in vitro. Journal of Biological Chemistry, 266 (34). pp. 22792-22695. ISSN 00219258 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1835972

Abstract

In nematodes, a fraction of mRNAs acquires a common 22-nucleotide 5'-terminal spliced leader sequence via a trans-splicing reaction. The same premessenger RNAs which receive the spliced leader are also processed by conventional cis-splicing. Whole cell extracts prepared from synchronous embryos of the parasitic nematode Ascaris lumbricoides catalyze both cis- and trans-splicing. We have used this cell-free system and oligodeoxynucleotide directed RNase H digestion to assess the U small nuclear RNA requirements for nematode cis- and trans-splicing. These experiments indicated that both cis- and trans-splicing require intact U2 and U4/U6 small nuclear ribonucleoproteins (sn-RNPs). However, whereas cis-splicing displays the expected requirement for an intact U1 snRNP, trans-splicing is unaffected when ~90% of U1 snRNP is degraded. These results suggest that 5' splice site identification differs in nematode cis- and trans-splicing.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: small nuclear ribonucleoprotein article ascaris lumbricoides nematode nonhuman priority journal rna splicing Animal Ascaris Base Sequence Electrophoresis, Polyacrylamide Gel Molecular Sequence Data Ribonucleoproteins Ribonucleoproteins, Small Nuclear RNA, Small Nuclear Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 1991
Date Deposited: 09 Jan 2013 17:35
Last Modified: 09 Jan 2013 17:35
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26499

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