Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolor

Haiser, H. J., Karginov, F. V., Hannon, G. J., Elliot, M. A. (2008) Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolor. Nucleic Acids Research, 36 (3). pp. 732-741. ISSN 03051048 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/18084030
DOI: 10.1093/nar/gkm1096

Abstract

The ability to sense and respond to environmental and physiological signals is critical for the survival of the soil-dwelling Gram-positive bacterium Streptomyces coelicolor. Nutrient deprivation triggers the onset of a complex morphological differentiation process that involves the raising of aerial hyphae and formation of spore chains, and coincides with the production of a diverse array of clinically relevant antibiotics and other secondary metabolites. These processes are tightly regulated; however, the genes and signals involved have not been fully elucidated. Here, we report a novel tRNA cleavage event that follows the same temporal regulation as morphological and physiological differentiation, and is growth medium dependent. All tRNAs appear to be susceptible to cleavage; however, there appears to be a bias towards increased cleavage of those tRNAs that specify highly utilized codons. In contrast to what has been observed in eukaryotes, accumulation of tRNA halves in S. coelicolor is not significantly affected by amino acid starvation, and is also not affected by induction of the stringent response or inhibition of ribosome function. Mutants defective in aerial development and antibiotic production exhibit altered tRNA cleavage profiles relative to wild-type strains. © 2007 The Author(s).

Item Type: Paper
Uncontrolled Keywords: amino acid bacterial RNA transfer RNA antibiotic biosynthesis article bacterial spore bacterial strain bacterial survival bacterium mutant codon controlled study culture medium Gram positive bacterium morphology nonhuman priority journal regulatory mechanism ribosome RNA cleavage signal transduction soil microflora Streptomyces coelicolor wild type Anticodon Cloning, Molecular Gene Expression Regulation, Bacterial Mutation Protein Synthesis Inhibitors Ribosomes RNA Processing, Post-Transcriptional RNA Stability RNA, Transfer Bacteria (microorganisms) Eukaryota Posibacteria
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
organism description > bacteria
CSHL Authors:
Communities: CSHL labs > Hannon lab
CSHL Post Doctoral Fellows
Depositing User: Matt Covey
Date: 2008
Date Deposited: 09 Jan 2013 16:53
Last Modified: 02 May 2013 19:27
PMCID: PMC2241913
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26448

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