The dynamics of a pre-mRNA splicing factor in living cells

Misteli, T., Cáceres, J. F., Spector, D. L. (1997) The dynamics of a pre-mRNA splicing factor in living cells. Nature, 387 (6632). pp. 523-527. ISSN 0028-0836

URL: http://www.ncbi.nlm.nih.gov/pubmed/9168118

Abstract

Pre-mRNA splicing is a predominantly co-transcriptional event which involves a large number of essential splicing factors. Within the mammalian cell nucleus, most splicing factors are concentrated in 20-40 distinct domains called speckles. The function of speckles and the organization of cellular transcription and pre-mRNA splicing in vivo are not well understood. We have investigated the dynamic properties of splicing factors in nuclei of living cells. Here we show that speckles are highly dynamic structures that respond specifically to activation of nearby genes. These dynamic events are dependent on RNA polymerase II transcription, and are sensitive to inhibitors of protein kinases and Ser/Thr phosphatases. When single genes are transcriptionally activated in living cells, splicing factors leave speckles in peripheral extensions and accumulate at the new sites of transcription. We conclude that one function of speckles is to supply splicing factors to active genes. Our observations demonstrate that the interphase nucleus is far more dynamic in nature than previously assumed.

Item Type: Paper
Uncontrolled Keywords: Cell Compartmentation Cell Line Cell Nucleus Green Fluorescent Proteins Luminescent Proteins Nuclear Proteins Phosphorylation RNA Polymerase II RNA Precursors RNA Splicing RNA-Binding Proteins Recombinant Fusion Proteins Transcription, Genetic
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 19:56
Last Modified: 29 Jan 2015 17:57
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26336

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