U1 and U2 small nuclear RNAs are present in nuclear speckles

Huang, S., Spector, D. L. (1992) U1 and U2 small nuclear RNAs are present in nuclear speckles. Proceedings of the National Academy of Sciences of the United States of America, 89 (1). pp. 305-308. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/1530887

Abstract

The localization of U1 and U2 small nuclear RNAs (snRNAs) has been examined by in situ hybridization using 2'-O-alkyl oligonucleotide probes. We have found that these snRNAs, which are essential for pre-mRNA splicing, localize in a speckled distribution, in addition to being present in three of four foci, in HeLa cell nuclei. However, in cells of defined passage, such as Detroit 551 and WI-38 fibroblasts, these snRNAs are concentrated in nuclear speckles, and foci are not observed. The speckled distribution of U1 and U2 snRNAs is coincident with the speckled regions enriched in small nuclear ribonucleoprotein particle (snRNP) proteins and the essential non-snRNP splicing factor SC-35. The localization of these key components of the pre-mRNA splicing machinery to speckled nuclear regions suggests that these regions may be involved in pre-mRNA splicing.

Item Type: Paper
Uncontrolled Keywords: Cell Compartmentation Cell Nucleus Fluorescent Antibody Technique HeLa Cells Humans Nuclear Proteins Nucleic Acid Hybridization RNA Splicing RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nuclear speckles
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 19:43
Last Modified: 29 Jan 2015 20:39
PMCID: PMC48225
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26318

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving