Nucleolar accumulation of poly (A)+ RNA in heat-shocked yeast cells: implication of nucleolar involvement in mRNA transport

Tani, T., Derby, R. J., Hiraoka, Y., Spector, D. L. (1995) Nucleolar accumulation of poly (A)+ RNA in heat-shocked yeast cells: implication of nucleolar involvement in mRNA transport. Molecular Biology of the Cell, 6 (11). pp. 1515-34. ISSN 1059-1524

URL: http://www.ncbi.nlm.nih.gov/pubmed/8589453

Abstract

Transport of mRNA from the nucleus to the cytoplasm plays an important role in gene expression in eukaryotic cells. In wild-type Schizosaccharomyces pombe cells poly(A)+ RNA is uniformly distributed throughout the nucleoplasm and cytoplasm. However, we found that a severe heat shock blocks mRNA transport in S. pombe, resulting in the accumulation of bulk poly(A)+ RNA, as well as a specific intron-less transcript, in the nucleoli. Pretreatment of cells with a mild heat shock, which induces heat shock proteins, before a severe heat shock protects the mRNA transport machinery and allows mRNA transport to proceed unimpeded. In heat-shocked S. pombe cells, the nucleolar region condensed into a few compact structures. Interestingly, poly(A)+ RNA accumulated predominantly in the condensed nucleolar regions of the heat-shocked cells. These data suggest that the yeast nucleolus may play a role in mRNA transport in addition to its roles in rRNA synthesis and preribosome assembly.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Cell Nucleolus drug effects metabolism ultrastructure Cycloheximide pharmacology Heat In Situ Hybridization Microscopy Electron Molecular Sequence Data Oligonucleotide Probes RNA Messenger metabolism Schizosaccharomyces metabolism ultrastructure
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Brian Soldo
Date Deposited: 30 Mar 2012 22:41
Last Modified: 29 Jan 2015 19:30
PMCID: PMC301308
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25770

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving