Methylation detection oligonucleotide microarray analysis: a high-resolution method for detection of CpG island methylation

Kamalakaran, S., Kendall, J. T., Zhao, X., Tang, C., Khan, S., Ravi, K., Auletta, T., Riggs, M., Wang, Y., Helland, A., Naume, Bj., Dimitrova, N., Borresen-Dale, A.. L., Hicks, J. B., Lucito, R. (2009) Methylation detection oligonucleotide microarray analysis: a high-resolution method for detection of CpG island methylation. Nucl. Acids Res., 37 (12). e89.

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URL: http://www.ncbi.nlm.nih.gov/pubmed/19474344
DOI: 10.1093/nar/gkp413

Abstract

Methylation of CpG islands associated with genes can affect the expression of the proximal gene, and methylation of non-associated CpG islands correlates to genomic instability. This epigenetic modification has been shown to be important in many pathologies, from development and disease to cancer. We report the development of a novel high-resolution microarray that detects the methylation status of over 25 000 CpG islands in the human genome. Experiments were performed to demonstrate low system noise in the methodology and that the array probes have a high signal to noise ratio. Methylation measurements between different cell lines were validated demonstrating the accuracy of measurement. We then identified alterations in CpG islands, both those associated with gene promoters, as well as non-promoter-associated islands in a set of breast and ovarian tumors. We demonstrate that this methodology accurately identifies methylation profiles in cancer and in principle it can differentiate any CpG methylation alterations and can be adapted to analyze other species.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA methylation
bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > CpG islands
CSHL Authors:
Communities: CSHL labs > Hicks lab
CSHL labs > Lucito lab
CSHL Cancer Center Shared Resources > Instrumentation Service
Depositing User: Brian Soldo
Date Deposited: 29 Mar 2012 14:37
Last Modified: 30 Dec 2014 16:54
PMCID: PMC2709589
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25538

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