RNA recognition and translational regulation by a homeodomain protein

Dubnau, J. T., Struhl, G. (February 1996) RNA recognition and translational regulation by a homeodomain protein. Nature, 379 (6567). pp. 694-9.

URL: http://www.ncbi.nlm.nih.gov/pubmed/8602214
DOI: 10.1038/379694a0

Abstract

In Drosophila, the primary determinant of anterior pattern is the gradient morphogen bicoid (bcd), a homeodomain protein that binds DNA and transcriptionally activates target genes at different threshold concentrations. Here we present evidence that bcd also binds RNA and acts as a translational repressor to generate an opposing gradient of the homeodomain protein caudal (cad). RNA binding by bcd seems to involve direct interactions between the bcd homeodomain and discrete target sequences within the 3' untranslated region of the cad messenger RNA and to block the initiation of cad translation.

Item Type: Paper
Uncontrolled Keywords: Animals Animals Genetically Modified Base Sequence Binding Sites Cell Line Dna Drosophila embryology genetics Drosophila Proteins Female Gene Expression Regulation Developmental Homeodomain Proteins genetics metabolism Insect Hormones metabolism Introns Molecular Sequence Data Peptide Chain Initiation Translational Peptide Fragments metabolism Protein Biosynthesis RNA Messenger metabolism RNA Binding Proteins metabolism Recombinant Fusion Proteins metabolism Regulatory Sequences Nucleic Acid Repressor Proteins metabolism Trans Activators Transcription Factors
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > RNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > translation
CSHL Authors:
Communities: CSHL labs > Dubnau lab
Highlight: Dubnau, Josh T.
Depositing User: Brian Soldo
Date: 22 February 1996
Date Deposited: 22 Mar 2012 16:58
Last Modified: 20 May 2014 15:53
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25431

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