Dubnau, J. T., Struhl, G. (February 1996) RNA recognition and translational regulation by a homeodomain protein. Nature, 379 (6567). pp. 694-9.
Abstract
In Drosophila, the primary determinant of anterior pattern is the gradient morphogen bicoid (bcd), a homeodomain protein that binds DNA and transcriptionally activates target genes at different threshold concentrations. Here we present evidence that bcd also binds RNA and acts as a translational repressor to generate an opposing gradient of the homeodomain protein caudal (cad). RNA binding by bcd seems to involve direct interactions between the bcd homeodomain and discrete target sequences within the 3' untranslated region of the cad messenger RNA and to block the initiation of cad translation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Animals Genetically Modified Base Sequence Binding Sites Cell Line Dna Drosophila embryology genetics Drosophila Proteins Female Gene Expression Regulation Developmental Homeodomain Proteins genetics metabolism Insect Hormones metabolism Introns Molecular Sequence Data Peptide Chain Initiation Translational Peptide Fragments metabolism Protein Biosynthesis RNA Messenger metabolism RNA Binding Proteins metabolism Recombinant Fusion Proteins metabolism Regulatory Sequences Nucleic Acid Repressor Proteins metabolism Trans Activators Transcription Factors |
| Subjects: | organism description > animal > insect > Drosophila bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > RNA binding protein bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > translation |
| CSHL Authors: | |
| Communities: | CSHL labs > Dubnau lab |
| Highlight: | Dubnau, Josh T. |
| Depositing User: | Brian Soldo |
| Date: | 22 February 1996 |
| Date Deposited: | 22 Mar 2012 16:58 |
| Last Modified: | 20 May 2014 15:53 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/25431 |
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