Viral-E1 and Viral-E2 Proteins Support Replication of Homologous and Heterologous Papillomaviral Origins

Chiang, C. M., Ustav, M., Stenlund, A., Ho, T. F., Broker, T. R., Chow, L. T. (July 1992) Viral-E1 and Viral-E2 Proteins Support Replication of Homologous and Heterologous Papillomaviral Origins. Proceedings of the National Academy of Sciences of the United States of America, 89 (13). pp. 5799-5803. ISSN 0027-8424

URL: https://www.ncbi.nlm.nih.gov/pubmed/1321423

Abstract

We have shown that E1 and E2 proteins of human papillomavirus type 11 (HPV-11) were essential to support the replication of the homologous viral origin (ori) in a transient replication assay, similar to reports on bovine papillomavirus type 1 (BPV-1). Unexpectedly, matched or even mixed combinations of E1 and E2 proteins from HPV-11 or BPV-1 replicated either ori in human, monkey, and rodent cell lines of epithelial or fibroblastic lineage, albeit with varied efficiencies. Either set of viral proteins was also able to initiate replication of ori-containing plasmids from many other human and animal papillomaviruses. Thus the interactions among the cis elements and trans factors of papillomaviruses are more conserved than expected from the other members of the papovavirus family, simian virus 40 and polyomavirus, for which large tumor antigen does not replicate a heterologous ori in either permissive or nonpermissive cells. We infer that the stringent species and tissue specificities observed for papillomaviruses in vivo are not entirely due to direct restrictions on viral DNA replication. Rather, transcriptional control of viral gene expression must play a dominant role.

Item Type: Paper
Uncontrolled Keywords: EUKARYOTIC DNA REPLICATION eukaryotic DNA replication HUMAN PAPILLOMAVIRUSES Human Papillomavirus REPLICATION FACTORS replication factor DNA POLYMERASE ALPHA polymerase alpha MESSENGER RNAS messenger RNA MOUSE CELLS mouse cells INITIATION initiation INVITRO in-vitro in vitro SYSTEM gene GENE
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > virus > papillomavirus
CSHL Authors:
Communities: CSHL labs > Stenlund lab
Depositing User: Brian Soldo
Date: July 1992
Date Deposited: 15 Mar 2012 15:09
Last Modified: 14 Feb 2017 20:36
PMCID: PMC402105
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25201

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