Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin

Shibahara, K., Stillman, B. (1999) Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Cell, 96 (4). pp. 575-85. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/10052459
DOI: 10.1016/S0092-8674(00)80661-3

Abstract

Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically determined chromosomal states in vivo and promotes assembly of chromatin during DNA replication in vitro. Herein, we demonstrate that after DNA replication, replicated, but not unreplicated, DNA is also competent for CAF-1-dependent chromatin assembly. The proliferating cell nuclear antigen (PCNA), a DNA polymerase clamp, is a component of the replication-dependent marking of DNA for chromatin assembly. The clamp loader, replication factor C (RFC), can reverse this mark by unloading PCNA from the replicated DNA. PCNA binds directly to p150, the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA replication in cells. We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and the inheritance of epigenetic chromosome states.

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphate pharmacology Autoantibodies pharmacology Cell Division genetics Chromatin genetics metabolism Chromosomal Proteins Non-Histone DNA Replication drug effects genetics DNA Superhelical drug effects DNA-Binding Proteins genetics metabolism pharmacology Hela Cells Homeodomain Proteins Humans Proliferating Cell Nuclear Antigen genetics immunology metabolism Protein Binding genetics Proto-Oncogene Proteins c-bcl-2 Replication Factor C Repressor Proteins Saccharomyces cerevisiae Proteins Simian virus 40/genetics Temperature
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 29 Feb 2012 19:57
Last Modified: 20 Jun 2017 19:24
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25052

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