The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencing

Bell, S. P., Mitchell, J., Leber, J., Kobayashi, R., Stillman, B. (1995) The multidomain structure of Orc1p reveals similarity to regulators of DNA replication and transcriptional silencing. Cell, 83 (4). pp. 563-8. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7585959
DOI: 10.1016/0092-8674(95)90096-9

Abstract

The origin recognition complex (ORC) is a six protein assembly that binds S. cerevisiae origins of replication and directs DNA replication throughout the genome and transcriptional silencing at the yeast mating-type loci. Here we report the cloning of the genes encoding the 120 kDa (ORC1), 62 kDa (ORC3), and 56 kDa (ORC4) subunits of ORC and the reconstitution of the complete complex after expression of all six subunits in insect cells. Orc1p is related to Cdc6p and Cdc18p, which regulate DNA replication and mitosis, and to Sir3p, a regulator of transcriptional silencing. The N-terminal region of Orc1p is highly related to Sir3p, and studies of Orc1p/Sir3p chimeric proteins indicate that this domain is dedicated to the transcriptional silencing function of ORC.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Cell Cycle Proteins genetics DNA Replication genetics DNA-Binding Proteins genetics isolation & purification ultrastructure Fungal Proteins genetics Genes Fungal genetics Genes Mating Type Fungal Molecular Sequence Data Origin Recognition Complex RNA Splicing genetics Repressor Proteins genetics isolation & purification ultrastructure Saccharomyces cerevisiae genetics Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Transcription, Genetic genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > origin recognition complex
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 07 Mar 2012 19:18
Last Modified: 20 Jun 2017 19:42
Related URLs:
URI: http://repository.cshl.edu/id/eprint/24938

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