RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs

Olson, A., Sheth, N., Lee, J. S., Hannon, G. J., Sachidanandam, R. (January 2006) RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs. Nucleic Acids Res, 34 (Databa). D153-7. ISSN 1362-4962 (Electronic)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/16381835
DOI: 10.1093/nar/gkj051

Abstract

Use of RNA interference (RNAi) in forward genetic screens is proliferating. Currently, short-interfering RNAs (siRNAs) and short-hairpin RNAs (shRNAs) are being used to silence genes to tease out functional information. It is becoming easier to harness RNAi to silence specific genes, owing to the development of libraries of readymade shRNA and siRNA gene-silencing constructs by using a variety of sources. RNAi Codex, which consists of a database of shRNA related information and an associated website, has been developed as a portal for publicly available shRNA resources and is accessible at http://codex.cshl.org. RNAi Codex currently holds data from the Hannon-Elledge shRNA library and allows the use of biologist-friendly gene names to access information on shRNA constructs that can silence the gene of interest. It is designed to hold user-contributed annotations and publications for each construct, as and when such data become available. We will describe features of RNAi Codex and explain the use of the tool.

Item Type: Paper
Uncontrolled Keywords: Animals Databases Nucleic Acid Humans Internet Mice RNA Interference RNA, Small Interfering chemistry Rats User-Computer Interface
Subjects: bioinformatics > genomics and proteomics > databases > database construction
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > databases > databases
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: CSHL Librarian
Date: 1 January 2006
Date Deposited: 09 Dec 2011 17:56
Last Modified: 08 Nov 2017 15:31
PMCID: PMC1347414
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22874

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