The reinforcement of invasion in epithelial ovarian cancer cells by 17?-Estradiol is associated with up-regulation of Snail

Ding, J. X., Feng, Y. J., Yao, L. Q., Yu, M., Jin, H. Y., Yin, L. H. (2006) The reinforcement of invasion in epithelial ovarian cancer cells by 17?-Estradiol is associated with up-regulation of Snail. Gynecologic Oncology, 103 (2). pp. 623-630. ISSN 00908258

URL: https://www.ncbi.nlm.nih.gov/pubmed/16806441
DOI: 10.1016/j.ygyno.2006.04.023

Abstract

Objective.: The transcription factor Snail, which is implicated in the triggering of epithelial-mesenchymal transitions (EMT), plays an important role in adhesion, invasion and metastasis of tumor cells. In the present study, we assessed 17?-Estradiol (E2)'s effect on Snail, E-cadherin and MMP-2 expression of epithelial ovarian cancer cell line ES-2 and SKOV3. Then we induced Snail gene silencing by RNA interference to explore the effect of E2 on E-cadherin and MMP-2 expression when Snail gene expression was blocked. Methods.: Treated by 10-8 M E2, Snail, E-cadherin and MMP-2 mRNA expression of the cells was measured by RT-PCR; Snail, MMP-2 protein expression was detected by IHC; and MMP-2 activity was determined by Zymography. E-cadherin protein level was measured by Western blot. We constructed the small interfering dsRNA expression vector (pRNAT-U6.1/Neo-Snail) targeting Snail gene, as well as a negative control vector (pRNAT-U6.1/Neo-Neg). Then the cells were transiently transfected with the vectors. Western blot and zymography were conducted to determine E-cadherin protein level and matrix metalloproteinase activity of the cells transfected with pRNAT-U6.1/Neo-Snail or pRNAT-U6.1/Neo-Neg after treated with E2 for 24 h. Results.: The expression of ER? mRNA and protein was negative in ES-2 cells and positive in SKOV3 cells, and ER? expression was positive in both cell lines. 10-8 mol/l E2 elevated expression of Snail and MMP-2 mRNA and protein in both ES-2 and SKOV3 cells, and reduced expression of E-cadherin mRNA and protein in SKOV3 cells. While in the RNAi group transfected with the small interfering dsRNA expression vector (pRNAT-U6.1/Neo-Snail) targeting Snail gene, E2 treatment did not have a significant effect on MMP-2 activity or E-cadherin protein in ES-2 and SKOV3 cells. Conclusions.: 17?-Estradiol increased Snail expression in both ER?-negative ES-2 cells and ER?-positive SKOV3 cells independent of the existence of ER?. The increase of MMP-2 expression in ES-2 and SKOV3 cells and decrease of E-cadherin expression in SKOV3 cells induced by E2 were associated with up-regulation of Snail. © 2006 Elsevier Inc. All rights reserved.

Item Type: Paper
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene silencing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: CSHL Librarian
Date: 2006
Date Deposited: 15 Dec 2011 16:48
Last Modified: 13 Apr 2018 15:21
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22784

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