Par6-aPKC uncouples ErbB2 induced disruption of polarized epithelial organization from proliferation control

Aranda, V., Haire, T., Nolan, M. E., Calarco, J. P., Rosenberg, A. Z., Fawcett, J. P., Pawson, T., Muthuswamy, S. K. (November 2006) Par6-aPKC uncouples ErbB2 induced disruption of polarized epithelial organization from proliferation control. Nat Cell Biol, 8 (11). pp. 1235-45. ISSN 1465-7392 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/17060907
DOI: 10.1038/ncb1485

Abstract

The polarized glandular organization of epithelial cells is frequently lost during development of carcinoma. However, the specific oncogene targets responsible for polarity disruption have not been identified. Here, we demonstrate that activation of ErbB2 disrupts apical-basal polarity by associating with Par6-aPKC, components of the Par polarity complex. Inhibition of interaction between Par6 and aPKC blocked the ability of ErbB2 to disrupt the acinar organization of breast epithelia and to protect cells from apoptosis but was not required for cell proliferation. Therefore, oncogenes target polarity proteins to disrupt glandular organization and protect cells from apoptotic death during development of carcinoma.

Item Type: Paper
Uncontrolled Keywords: Animals Apoptosis physiology Carrier Proteins metabolism Cell Cycle physiology Cell Line Cell Polarity physiology Cell Proliferation Epithelial Cells cytology metabolism Gene Expression Immunoblotting Immunoprecipitation Microscopy Fluorescence Protein Binding Protein Kinase C metabolism Receptor erbB-2 genetics metabolism Signal Transduction physiology
Subjects: diseases & disorders > cancer
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
organs, tissues, organelles, cell types and functions > organs types and functions > breast
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell polarity
organs, tissues, organelles, cell types and functions > tissues types and functions > epithelia
CSHL Authors:
Communities: CSHL labs > Muthuswamy lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: November 2006
Date Deposited: 20 Dec 2011 18:27
Last Modified: 26 Sep 2014 15:54
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22750

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