Regulating gene expression through RNA nuclear retention

Prasanth, K. V. , Prasanth, S. G., Xuan, Z. Y., Hearn, S., Freier, S. M., Bennett, C. F., Zhang, M. Q., Spector, D. L. (2005) Regulating gene expression through RNA nuclear retention. Cell, 123 (2). pp. 249-263. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/16239143
DOI: 10.1016/j.cell.2005.08.033

Abstract

Multiple mechanisms have evolved to regulate the eukaryotic genome. We have identified CTN-RNA, a mouse tissue-specific similar to 8 kb nuclear-retained poly(A)(+) RNA that regulates the level of its protein-coding partner. CTN-RNA is transcribed from the protein-coding mouse cationic amino acid transporter 2 (mCAT2) gene through alternative promoter and poly(A) site usage. CTN-RNA is diffusely distributed in nuclei and is also localized to paraspeckles. The 3'UTR of CTN-RNA contains elements for adenosine-to-inosine editing, involved in its nuclear retention. Interestingly, knockdown of CTN-RNA also downregulates mCAT2 mRNA. Under stress, CTN-RNA is posttranscriptionally cleaved to produce protein-coding mCAT2 mRNA. Our findings reveal a role of the cell nucleus in harboring RNA molecules that are not immediately needed to produce proteins but whose cytoplasmic presence is rapidly required upon physiologic stress. This mechanism of action highlights an important paradigm for the role of a nuclear-retained stable RNA transcript in regulating gene expression.

Item Type: Paper
Uncontrolled Keywords: interchromatin granule clusters nitric oxide synthase in-vivo ANALYSIS messenger RNA translational control human transcriptome HSR-Omega adenosine deaminases noncoding RNA poly (a) RNA Poly (A) RNA CTN-RNA
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > Mapping and Rendering > RNA Structure Rendering
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA replication
CSHL Authors:
Communities: CSHL labs > Spector lab
CSHL labs > Zhang lab
Depositing User: CSHL Librarian
Date Deposited: 06 Jan 2012 14:33
Last Modified: 28 Jan 2015 22:06
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22677

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving