Normal microRNA maturation and germ-line stem cell maintenance requires loquacious, a double-stranded RNA- binding domain protein

Forstemann, K., Tomari, Y., Du, T. T., Vagin, V. V., Denli, A. M., Bratu, D. P., Klattenhoff, C., Theurkauf, W. E., Zamore, P. D. (July 2005) Normal microRNA maturation and germ-line stem cell maintenance requires loquacious, a double-stranded RNA- binding domain protein. Plos Biology, 3 (7). pp. 1187-1201. ISSN 1544-9173

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URL: http://www.ncbi.nlm.nih.gov/pubmed/15918770
DOI: 10.1371/journal.pbio.0030236

Abstract

microRNAs ( miRNAs) are single-stranded, 21- to 23-nucleotide cellular RNAs that control the expression of cognate target genes. Primary miRNA (pri-miRNA) transcripts are transformed to mature miRNA by the successive actions of two RNase III endonucleases. Drosha converts pri-miRNA transcripts to precursor miRNA (pre-miRNA); Dicer, in turn, converts pre-miRNA to mature miRNA. Here, we show that normal processing of Drosophila pre-miRNAs by Dicer-1 requires the double-stranded RNA-binding domain (dsRBD) protein Loquacious (Loqs), a homolog of human TRBP, a protein first identified as binding the HIV trans-activator RNA ( TAR). Efficient miRNA-directed silencing of a reporter transgene, complete repression of white by a dsRNA trigger, and silencing of the endogenous Stellate locus by Suppressor of Stellate, all require Loqs. In loqs f(00791) mutant ovaries, germ-line stem cells are not appropriately maintained. Loqs associates with Dcr-1, the Drosophila RNase III enzyme that processes pre-miRNA into mature miRNA. Thus, every known Drosophila RNase-III endonuclease is paired with a dsRBD protein that facilitates its function in small RNA biogenesis.

Item Type: Paper
Uncontrolled Keywords: DROSOPHILA-MELANOGASTER Drosophila Melanogaster NUCLEAR EXPORT nuclear export C-ELEGANS C-elagans C elegans MICROPROCESSOR microprocessor COMPLEX ARABIDOPSIS-THALIANA Arabidopsis thaliana Arabidopsis Thaliana OOCYTE DETERMINATION oocyte determination CRYSTAL-STRUCTURE crystal structure SLICER ACTIVITY slicer activity GENE-REGULATION gene regulation DISTINCT ROLES distinct roles
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: July 2005
Date Deposited: 13 Jan 2012 18:48
Last Modified: 25 Sep 2014 20:43
PMCID: PMC1141267
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22570

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