Forstemann, K., Tomari, Y., Du, T. T., Vagin, V. V., Denli, A. M., Bratu, D. P., Klattenhoff, C., Theurkauf, W. E., Zamore, P. D. (July 2005) Normal microRNA maturation and germ-line stem cell maintenance requires loquacious, a double-stranded RNA- binding domain protein. Plos Biology, 3 (7). pp. 1187-1201. ISSN 1544-9173
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Abstract
microRNAs ( miRNAs) are single-stranded, 21- to 23-nucleotide cellular RNAs that control the expression of cognate target genes. Primary miRNA (pri-miRNA) transcripts are transformed to mature miRNA by the successive actions of two RNase III endonucleases. Drosha converts pri-miRNA transcripts to precursor miRNA (pre-miRNA); Dicer, in turn, converts pre-miRNA to mature miRNA. Here, we show that normal processing of Drosophila pre-miRNAs by Dicer-1 requires the double-stranded RNA-binding domain (dsRBD) protein Loquacious (Loqs), a homolog of human TRBP, a protein first identified as binding the HIV trans-activator RNA ( TAR). Efficient miRNA-directed silencing of a reporter transgene, complete repression of white by a dsRNA trigger, and silencing of the endogenous Stellate locus by Suppressor of Stellate, all require Loqs. In loqs f(00791) mutant ovaries, germ-line stem cells are not appropriately maintained. Loqs associates with Dcr-1, the Drosophila RNase III enzyme that processes pre-miRNA into mature miRNA. Thus, every known Drosophila RNase-III endonuclease is paired with a dsRBD protein that facilitates its function in small RNA biogenesis.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | DROSOPHILA-MELANOGASTER Drosophila Melanogaster NUCLEAR EXPORT nuclear export C-ELEGANS C-elagans C elegans MICROPROCESSOR microprocessor COMPLEX ARABIDOPSIS-THALIANA Arabidopsis thaliana Arabidopsis Thaliana OOCYTE DETERMINATION oocyte determination CRYSTAL-STRUCTURE crystal structure SLICER ACTIVITY slicer activity GENE-REGULATION gene regulation DISTINCT ROLES distinct roles |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab School of Biological Sciences > Publications |
| Depositing User: | CSHL Librarian |
| Date: | July 2005 |
| Date Deposited: | 13 Jan 2012 18:48 |
| Last Modified: | 25 Sep 2014 20:43 |
| PMCID: | PMC1141267 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22570 |
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