miRNAs on the move: miRNA biogenesis and the RNAi machinery

Murchison, E. P., Hannon, G. J. (June 2004) miRNAs on the move: miRNA biogenesis and the RNAi machinery. Curr Opin Cell Biol, 16 (3). pp. 223-9. ISSN 0955-0674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/15145345
DOI: 10.1016/j.ceb.2004.04.003

Abstract

Recent advances have led to a more detailed understanding of RNA interference and its role in microRNA biogenesis and function. Primary microRNA transcripts are processed by the RNaseIII nuclease, Drosha, and are exported from the nucleus by Exportin-5. Dicer cleaves microRNAs into their mature forms, which can be incorporated into effector complexes that mediate gene silencing activities. The 3' two-nucleotide overhang structure, a signature of RNaseIII cleavage, has been identified as a critical specificity determinant in targeting and maintaining small RNAs in the RNA interference pathway. MicroRNA functional analyses and genetic and biochemical interrogation of components of the pathway are starting to provide a glimpse at the range of biological processes and phenomena regulated by RNA interference.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Nucleus metabolism Cytoplasm metabolism Humans MicroRNAs genetics metabolism RNA Interference RNA Transport RNA-Induced Silencing Complex
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: June 2004
Date Deposited: 31 Jan 2012 16:23
Last Modified: 19 Sep 2014 15:06
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22446

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