Pattern formation in leaves via small RNA mobility

Chitwood, D. H., Nogueira, F. T., Howell, M. D., Montogmery, T. A., Carrington, J. C., Timmermans, M. C. (2008) Pattern formation in leaves via small RNA mobility. Developmental Biology, 319 (2). pp. 587-588. ISSN 0012-1606

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DOI: 10.1016/j.ydbio.2008.05.479


Small RNAs, including microRNAs (miRNAs) and trans-acting short interfering RNAs (ta-siRNAs), have important regulatory roles in development. In plants, members of these classes of small RNAs act to pattern the adaxial–abaxial (dorsal–ventral) axis of leaves. The AUXIN-RESPONSE FACTOR (ARF) family members ARF3 and ARF4 are together necessary to establish abaxial (ventral) fate in leaves. ARF3 and ARF4 are targets of ta-siRNAs that are termed “tasiR-ARFs.” To begin to understand the possible role of tasiR-ARFs in leaf polarity, we have localized the biogenesis components of the tasiR-ARF pathway, including the microRNA miR390, the activity of the ARGONAUTE gene required for miR390 activity (AGO7), and the activity of tasiR-ARFs themselves. We provide evidence that the tasiR-ARF pathway in Arabidopsis acts non-cell autonomously to maintain the polarized accumulation of ARF3 in leaf primordia. Small RNAs (both miR390 and tasiR-ARFs) in this specialized RNAi pathway may contribute to its non-cell autonomous activity, as they accumulate outside their discrete regions of biogenesis. We propose that small RNAs can possibly function as mobile inductive signals to direct patterning events during development

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > analysis and processing > NETBAG
organism description > plant behavior
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
CSHL Authors:
Communities: CSHL labs > Timmermans lab
School of Biological Sciences > Publications
Depositing User: Tom Adams
Date: 2008
Date Deposited: 25 Aug 2011 18:48
Last Modified: 26 Sep 2014 19:39
PMCID: PMC2658522
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