Chitwood, D. H., Nogueira, F. T., Howell, M. D., Montogmery, T. A., Carrington, J. C., Timmermans, M. C. (2008) Pattern formation in leaves via small RNA mobility. Developmental Biology, 319 (2). pp. 587-588. ISSN 0012-1606
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Abstract
Small RNAs, including microRNAs (miRNAs) and trans-acting short interfering RNAs (ta-siRNAs), have important regulatory roles in development. In plants, members of these classes of small RNAs act to pattern the adaxial–abaxial (dorsal–ventral) axis of leaves. The AUXIN-RESPONSE FACTOR (ARF) family members ARF3 and ARF4 are together necessary to establish abaxial (ventral) fate in leaves. ARF3 and ARF4 are targets of ta-siRNAs that are termed “tasiR-ARFs.” To begin to understand the possible role of tasiR-ARFs in leaf polarity, we have localized the biogenesis components of the tasiR-ARF pathway, including the microRNA miR390, the activity of the ARGONAUTE gene required for miR390 activity (AGO7), and the activity of tasiR-ARFs themselves. We provide evidence that the tasiR-ARF pathway in Arabidopsis acts non-cell autonomously to maintain the polarized accumulation of ARF3 in leaf primordia. Small RNAs (both miR390 and tasiR-ARFs) in this specialized RNAi pathway may contribute to its non-cell autonomous activity, as they accumulate outside their discrete regions of biogenesis. We propose that small RNAs can possibly function as mobile inductive signals to direct patterning events during development
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > analysis and processing > NETBAG organism description > plant behavior bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins |
| CSHL Authors: | |
| Communities: | CSHL labs > Timmermans lab School of Biological Sciences > Publications |
| Depositing User: | Tom Adams |
| Date: | 2008 |
| Date Deposited: | 25 Aug 2011 18:48 |
| Last Modified: | 26 Sep 2014 19:39 |
| PMCID: | PMC2658522 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/7708 |
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