Castle, J. C., Zhang, C., Shah, J. K., Kulkarni, A.t V., Kalsotra, A., Cooper, T. A., Johnson, J. M. (2008) Expression of 24,426 human alternative splicing events and predicted cis regulation in 48 tissues and cell lines. Nat Genet, 40 (12). pp. 1416-1425.
Abstract
partially because of the limited scale at which the expression of specific splicing events has been measured. We generated the first genome-scale expression compendium of human alternative splicing events using custom whole-transcript microarrays monitoring expression of 24,426 alternative splicing events in 48 diverse human samples. Over 11,700 genes and 9,500 splicing events were differentially expressed, providing a rich resource for studying splicing regulation. An unbiased, systematic screen of 21,760 4-mer to 7-mer words for cis-regulatory motifs identified 143 RNA 'words' enriched near regulated cassette exons, including six clusters of motifs represented by UCUCU, UGCAUG, UGCU, UGUGU, UUUU and AGGG, which map to trans-acting regulators PTB, Fox, Muscleblind, CELF/CUG-BP, TIA-1 and hnRNP F/H, respectively. Each cluster showed a distinct pattern of genomic location and tissue specificity. For example, UCUCU occurs 110 to 35 nucleotides preceding cassette exons upregulated in brain and striated muscle but depleted in other tissues. UCUCU and UGCAUG seem to have similar function but independent action, occurring 5' and 3', respectively, of 33% of the cassette exons upregulated in skeletal muscle but co-occurring for only 2%.
| Item Type: | Paper |
|---|---|
| Additional Information: | 1061-4036 10.1038/ng.264c DOI 10.1038/ng.264 |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > analysis and processing > NETBAG |
| CSHL Authors: | |
| Communities: | CSHL labs > Zhang lab |
| Depositing User: | Tom Adams |
| Date: | 2008 |
| Date Deposited: | 16 Aug 2011 14:20 |
| Last Modified: | 13 Mar 2018 20:25 |
| PMCID: | PMC3197713 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/7700 |
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