Zali, Narges, El Demerdash, Osama, Chougule, Kapeel, Lu, Zhenyuan, Ware, Doreen, Stillman, Bruce (December 2025) Genome sequence assembly and annotation of MATA and MATB strains of Yarrowia lipolytica. NAR Genomics and Bioinformatics, 7 (4). lqaf175. ISSN 2631-9268 (Public Dataset)
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Abstract
Yeast is commonly utilized in molecular and cell biology research, and Yarrowia lipolytica is favored by bioengineers due to its ability to produce copious amounts of lipids, chemicals, and enzymes for industrial applications. Y. lipolytica is a dimorphic yeast that can proliferate in aerobic and hydrophobic environments conducive to industrial use. However, there is limited knowledge about the basic molecular biology of this yeast, including how the genome is duplicated and how gene silencing occurs. Genome sequences of Y. lipolytica strains have offered insights into this yeast species and have facilitated the development of new industrial applications. Although previous studies have reported the genome sequence of a few Y. lipolytica strains, it is of value to have more precise sequences and annotation, particularly for studies of the biology of this yeast. To further study and characterize the molecular biology of this microorganism, a high-quality reference genome assembly and annotation has been produced for two related Y. lipolytica strains of the opposite mating type, strain E122 (MATA) and 22301-5 (MATB). The combination of short-read and long-read sequencing of genome DNA and short-read and long-read sequencing of transcript cDNAs allowed the genome assembly and a comparison with a distantly related Yarrowia strain.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > annotation bioinformatics bioinformatics > genomics and proteomics |
| CSHL Authors: | |
| Communities: | CSHL Cancer Center Program > Gene Regulation and Inheritance Program CSHL labs > Stillman lab CSHL labs > Ware lab |
| SWORD Depositor: | CSHL Elements |
| Highlight: | Stillman, Bruce W. |
| Depositing User: | CSHL Elements |
| Date: | December 2025 |
| Date Deposited: | 18 Dec 2025 14:16 |
| Last Modified: | 18 Dec 2025 14:16 |
| PMCID: | PMC12693490 |
| Related URLs: | |
| Dataset ID: |
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| URI: | https://repository.cshl.edu/id/eprint/42039 |
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