Xavier, Andre M, Lin, Qianyu, Kang, Chris J, Cheadle, Lucas (February 2025) A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex. Development. dev.204244. ISSN 0950-1991 (Public Dataset)
![[thumbnail of 10.1242.dev.204244.pdf]](https://repository.cshl.edu/style/images/fileicons/application_pdf.png) |
PDF
10.1242.dev.204244.pdf - Published Version Available under License Creative Commons Attribution. Download (17MB) |
Abstract
Sensory experience drives the maturation of neural circuits during postnatal brain development through molecular mechanisms that remain to be fully elucidated. One likely mechanism involves the sensory-dependent expression of genes that encode direct mediators of circuit remodeling within developing cells. To identify potential drivers of sensory-dependent synaptic development, we generated a single-nucleus RNA sequencing dataset describing the transcriptional responses of cells in mouse visual cortex to sensory deprivation or stimulation during a developmental window when visual input is necessary for circuit refinement. We sequenced 118,529 nuclei across sixteen neuronal and non-neuronal cell types isolated from control, sensory deprived, and sensory stimulated mice, identifying 1,268 sensory-induced genes within the developing brain. While experience elicited transcriptomic changes in all cell types, excitatory neurons in layer 2/3 exhibited the most robust changes, and the sensory-induced genes in these cells are poised to strengthen synapse-to-nucleus crosstalk and to promote cell-type-specific axon guidance pathways. Altogether, we expect this dataset to significantly broaden our understanding of the molecular mechanisms through which sensory experience shapes neural circuit wiring in the developing brain.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics organism description > animal organism description > animal > mammal organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent organs, tissues, organelles, cell types and functions > tissues types and functions bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transcriptomes organs, tissues, organelles, cell types and functions > tissues types and functions > visual cortex |
| CSHL Authors: | |
| Communities: | CSHL labs > Cheadle lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 28 February 2025 |
| Date Deposited: | 27 Mar 2025 18:25 |
| Last Modified: | 27 Mar 2025 18:25 |
| Related URLs: | |
| Dataset ID: | |
| URI: | https://repository.cshl.edu/id/eprint/41837 |
Actions (login required)
 |
Administrator's edit/view item |
