Antagonistic kinesin-14s within a single chromosomal drive haplotype

Brady, Meghan J, Gupta, Anjali, Gent, Jonathan I, Swentowsky, Kyle W, Unckless, Robert L, Dawe, R Kelly (February 2025) Antagonistic kinesin-14s within a single chromosomal drive haplotype. bioRxiv. ISSN 2692-8205 (Public Dataset) (Submitted)

PDF 10.1101.2025.02.05.636711.pdf - Submitted Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (8MB)

Abstract

In maize, there are two meiotic drive systems that operate on large tandem repeat arrays called knobs that are found on chromosome arms. One meiotic drive haplotype, Abnormal chromosome 10 (Ab10), encodes two kinesin proteins that interact with two distinct tandem repeat arrays in a sequence-specific manner to confer meiotic drive. The kinesin KINDR associates with knob180 repeats while the kinesin TRKIN associates with TR-1 repeats. Prior data show that meiotic drive is conferred primarily by the KINDR/knob180 system, with the TRKIN/TR-1 system having little or no role. The second meiotic drive haplotype, K10L2, shows low levels of meiotic drive and only encodes the TRKIN/TR-1 system. Here we used long-read sequencing to assemble the K10L2 haplotype and showed that it has strong homology to an internal portion of the Ab10 haplotype. We also carried out CRISPR mutagenesis of Trkin to test the role of Trkin on Ab10 and K10L2. The data indicate that the Trkin gene on Ab10 does not improve drive or fitness but instead has a weak deleterious effect when paired with a normal chromosome 10. The deleterious effect is more severe when Ab10 is paired with K10L2: in this context functional Trkin on either chromosome nearly abolishes Ab10 drive. We modeled the effect of Trkin on Ab10 and found it should not persist in the population. We conclude that Trkin either confers an advantage to Ab10 in untested circumstances or that it is in the process of being purged from the Ab10 population.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics > heterochromatin
CSHL Authors:	Swentowsky, Kyle
Communities:	CSHL labs > Jackson lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	7 February 2025
Date Deposited:	27 Feb 2025 20:22
Last Modified:	27 Feb 2025 20:22
PMCID:	PMC11839137
Related URLs:	Publisher
Dataset ID:	https://github.com/dawelab/TRKIN_Published.git
URI:	https://repository.cshl.edu/id/eprint/41802

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