Colonna, Marco, Konopka, Genevieve, Liddelow, Shane A, Nowakowski, Tomasz, Awatramani, Rajeshwar, Bateup, Helen S, Cadwell, Cathryn R, Caglayan, Emre, Chen, Jerry L, Gillis, Jesse, Kampmann, Martin, Krienen, Fenna, Marsh, Samuel E, Monje, Michelle, O'Dea, Michael R, Patani, Rickie, Pollen, Alex A, Quintana, Francisco J, Scavuzzo, Marissa, Schmitz, Matthew, Sloan, Steven A, Tesar, Paul J, Tollkuhn, Jessica, Tosches, Maria Antonietta, Urbanek, Madeleine E, Werner, Jonathan M, Bayraktar, Omer A, Gokce, Ozgun, Habib, Naomi (December 2024) Implementation and validation of single-cell genomics experiments in neuroscience. Nature Neuroscience, 27 (12). pp. 2310-2325. ISSN 1097-6256
Abstract
Single-cell or single-nucleus transcriptomics is a powerful tool for identifying cell types and cell states. However, hypotheses derived from these assays, including gene expression information, require validation, and their functional relevance needs to be established. The choice of validation depends on numerous factors. Here, we present types of orthogonal and functional validation experiment to strengthen preliminary findings obtained using single-cell and single-nucleus transcriptomics as well as the challenges and limitations of these approaches.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics organism description > animal organism description > animal > mammal > primates > hominids organism description > animal > mammal > primates > hominids > human organism description > animal > mammal organism description > animal > mammal > primates |
| CSHL Authors: | |
| Communities: | CSHL labs > Gillis Lab CSHL labs > Tollkuhn lab School of Biological Sciences > Publications |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | December 2024 |
| Date Deposited: | 06 Dec 2024 18:29 |
| Last Modified: | 09 Dec 2024 20:56 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41755 |
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