Simultaneous collection of in vivo functional and anatomical data from individual neurons in awake mice

Cazakoff, BN, Shea, SD (January 2015) Simultaneous collection of in vivo functional and anatomical data from individual neurons in awake mice. In: Neural Tracing Methods. UNSPECIFIED, pp. 85-99.

URL: http://doi.org/10.1007/978-1-4939-1963-5_4

DOI: 10.1007/978-1-4939-1963-5_4

Abstract

Ideally, to trace neural circuits, one often desires access to functional data that may be linked to anatomical attributes such as neuron type or projection target. Here we describe methods used for this purpose in our laboratory. We aim for this chapter to serve as a practical guide to applying “loose patch” or “cell-attached patch” electrophysiology techniques in vivo to simultaneously obtain information about neuronal fi ring (e.g., responses to sensory stimuli) and detailed anatomical information including dendritic morphology and axonal targeting. Since data on neuronal circuit function are often most useful when gathered during wakeful behavior, we pay special attention here to the use of these techniques in awake, head-fi xed mice. However, the same methods may easily be applied to anesthetized animals.

Item Type:	Book Section
Subjects:	organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions organism description > animal > mammal organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent
CSHL Authors:	Shea, Stephen D. Cazakoff, Brittany
Communities:	CSHL labs > Shea lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	1 January 2015
Date Deposited:	28 Oct 2024 19:18
Last Modified:	28 Oct 2024 19:18
URI:	https://repository.cshl.edu/id/eprint/41719

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