Metabotropic signaling within somatostatin interneurons controls transient thalamocortical inputs during development

Dwivedi, Deepanjali, Dumontier, Dimitri, Sherer, Mia, Lin, Sherry, Mirow, Andrea MC, Qiu, Yanjie, Xu, Qing, Liebman, Samuel A, Joseph, Djeckby, Datta, Sandeep R, Fishell, Gord, Pouchelon, Gabrielle (June 2024) Metabotropic signaling within somatostatin interneurons controls transient thalamocortical inputs during development. Nature Communications, 15 (1). p. 5421. ISSN 2041-1723 (Public Dataset)

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Abstract

During brain development, neural circuits undergo major activity-dependent restructuring. Circuit wiring mainly occurs through synaptic strengthening following the Hebbian "fire together, wire together" precept. However, select connections, essential for circuit development, are transient. They are effectively connected early in development, but strongly diminish during maturation. The mechanisms by which transient connectivity recedes are unknown. To investigate this process, we characterize transient thalamocortical inputs, which depress onto somatostatin inhibitory interneurons during development, by employing optogenetics, chemogenetics, transcriptomics and CRISPR-based strategies in mice. We demonstrate that in contrast to typical activity-dependent mechanisms, transient thalamocortical connectivity onto somatostatin interneurons is non-canonical and involves metabotropic signaling. Specifically, metabotropic-mediated transcription, of guidance molecules in particular, supports the elimination of this connectivity. Remarkably, we found that this process impacts the development of normal exploratory behaviors of adult mice.

Item Type:	Paper
Subjects:	organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons organs, tissues, organelles, cell types and functions > cell types and functions > cell types > interneurons organism description > animal > mammal organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:	Pouchelon, Gabrielle Dumontier, Dimitri Mirow, Andrea Liebman, Samuel Joseph, Djeckby
Communities:	CSHL labs > Pouchelon lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	26 June 2024
Date Deposited:	01 Jul 2024 17:49
Last Modified:	01 Jul 2024 17:49
PMCID:	PMC11208423
Related URLs:	Publisher
Dataset ID:	GEO: GSE165233 GEO: GSE104156 https://doi.org/10.5281/zenodo.11074522
URI:	https://repository.cshl.edu/id/eprint/41590

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