Williams, Jason, Nash, Bruce, Ghiban, Cornel, Khalfan, Mohammed, Hilgert, Uwe, Lauter, Susan, Yang, Chun-Hua, Micklos, David Andrew (April 2024) Analysis of DNA Barcodes Using DNA Subway. Methods in molecular biology (Clifton, N.J.), 2744. pp. 551-560. ISSN 1064-3745
Abstract
DNA Subway makes bioinformatic analysis of DNA barcodes classroom friendly, eliminating the need for software installations or command line tools. Subway bundles research-grade bioinformatics software into workflows with an easy-to-use interface. This chapter covers DNA Subway's DNA barcoding analysis workflow (Blue Line) starting with one or more Sanger sequence reads. During analysis, users can view trace files and sequence quality, pair and align forward and reverse reads, create and trim consensus sequences, perform BLAST searches, select reference data, align multiple sequences, and compute phylogenetic trees. High-quality sequences with the required metadata can also be submitted as barcode sequences to NCBI GenBank.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics Investigative techniques and equipment Investigative techniques and equipment > assays bioinformatics > computational biology |
| CSHL Authors: | |
| Communities: | Dolan DNA Learning Center |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 30 April 2024 |
| Date Deposited: | 06 May 2024 13:36 |
| Last Modified: | 06 May 2024 13:36 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41525 |
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