Oblique light-sheet tomography: fast and high resolution volumetric imaging of mouse brains

Narasimhan, Arun, Venkataraju, Kannan Umadevi, Mizrachi, Judith, Albeanu, Dinu F, Osten, Pavel (April 2017) Oblique light-sheet tomography: fast and high resolution volumetric imaging of mouse brains. bioRxiv. (Submitted)

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Abstract

Present light sheet fluorescence microscopes lack the wherewithal to image the whole brain (large tissues) with uniform illumination/detection parameters and high enough resolution to provide an understanding of the various aspects of neuroanatomy. To overcome this, we developed an oblique version of the light sheet microscope (Oblique Light Sheet Tomography, OLST) that includes a high magnification objective and serial sectioning, for volumetric imaging of the whole mouse brain at high spatial resolution at constant illumination/detection. We developed a novel gelatin based re-embedding procedure that makes the cleared brain rigid so that it can sectioned using our integrated microtome. Here, we characterize OLST and show that it can be used to observe dendritic morphology, spines and follow axons over a few mm in the mouse brain.

Item Type:	Paper
Subjects:	organism description > animal organs, tissues, organelles, cell types and functions > organs types and functions > brain organism description > animal > mammal organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions > organs types and functions organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent
CSHL Authors:	Narasimhan, Arun Albeanu, Dinu F. Osten, Pavel Umadevi Venkataraju, Kannan Mizrachi, Judith
Communities:	CSHL labs > Albeanu lab CSHL labs > Osten lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	30 April 2017
Date Deposited:	20 Dec 2023 20:38
Last Modified:	20 Dec 2023 20:38
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/41355

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