A technology platform for standardized cryoprotection and freezing of large-volume brain tissues for high-resolution histology

Kumarasami, Ramdayalan, Verma, Richa, Pandurangan, Karthika, Ramesh, Jivitha Jyothi, Pandidurai, Sathish, Savoia, Stephen, Jayakumar, Jaikishan, Bota, Mihail, Mitra, Partha, Joseph, Jayaraj, Sivaprakasam, Mohanasankar (2023) A technology platform for standardized cryoprotection and freezing of large-volume brain tissues for high-resolution histology. Frontiers in Neuroanatomy, 17. p. 1292655. ISSN 1662-5129

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Abstract

Understanding and mapping the human connectome is a long-standing endeavor of neuroscience, yet the significant challenges associated with the large size of the human brain during cryosectioning remain unsolved. While smaller brains, such as rodents and marmosets, have been the focus of previous connectomics projects, the processing of the larger human brain requires significant technological advancements. This study addresses the problem of freezing large brains in aligned neuroanatomical coordinates with minimal tissue damage, facilitating large-scale distortion-free cryosectioning. We report the most effective and stable freezing technique utilizing an appropriate choice of cryoprotection and leveraging engineering tools such as brain master patterns, custom-designed molds, and a continuous temperature monitoring system. This standardized approach to freezing enables high-quality, distortion-free histology, allowing researchers worldwide to explore the complexities of the human brain at a cellular level. Our approach combines neuroscience and engineering technologies to address this long-standing challenge with limited resources, enhancing accessibility of large-scale scientific endeavors beyond developed countries, promoting diverse approaches, and fostering collaborations.

Item Type:	Paper
Subjects:	Investigative techniques and equipment Investigative techniques and equipment > electrophysiology neurobiology neurobiology > neuroscience
CSHL Authors:	Savoia, Stephen Mitra, Partha P.
Communities:	CSHL labs > Mitra lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	2023
Date Deposited:	20 Dec 2023 16:44
Last Modified:	10 Jan 2024 20:03
PMCID:	PMC10651725
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/41325

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