High-throughput sequencing of macaque basolateral amygdala projections reveals dissociable connectional motifs with frontal cortex

Zeisler, Zachary R, London, Liza, Janssen, William G, Fredericks, J Megan, Elorette, Catherine, Fujimoto, Atsushi, Zhan, Huiqing, Russ, Brian E, Clem, Roger L, Hof, Patrick R, Stoll, Frederic M, Rudebeck, Peter H (February 2023) High-throughput sequencing of macaque basolateral amygdala projections reveals dissociable connectional motifs with frontal cortex. bioRxiv.

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Abstract

The basolateral amygdala (BLA) projects widely across the macaque frontal cortex1-4, and amygdalo-frontal projections are critical for optimal emotional responding5 and decision-making6. Yet, little is known about the single-neuron architecture of these projections: namely, whether single BLA neurons project to multiple parts of the frontal cortex. Here, we use MAPseq7 to determine the projection patterns of over 3000 macaque BLA neurons. We found that one-third of BLA neurons have two or more distinct targets in parts of frontal cortex and of subcortical structures. Further, we reveal non-random structure within these branching patterns such that neurons with four targets are more frequently observed than those with two or three, indicative of widespread networks. Consequently, these multi-target single neurons form distinct networks within medial and ventral frontal cortex consistent with their known functions in regulating mood and decision-making. Additionally, we show that branching patterns of single neurons shape functional networks in the brain as assessed by fMRI-based functional connectivity. These results provide a neuroanatomical basis for the role of the BLA in coordinating brain-wide responses to valent stimuli8 and highlight the importance of high-resolution neuroanatomical data for understanding functional networks in the brain.

Item Type:	Paper
Subjects:	organism description > animal organism description > animal > mammal organism description > animal > mammal > primates
CSHL Authors:	Zhan, Huiqing
Communities:	CSHL labs > Zador lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	20 February 2023
Date Deposited:	01 Nov 2023 18:52
Last Modified:	01 Nov 2023 18:52
PMCID:	PMC9882200
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/41296

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