Automated placement of stereotactic injections using a laser scan of the skull

Henderson, Margaret, Pinskiy, Vadim, Tolpygo, Alexander, Savoia, Stephen, Grange, Pascal, Mitra, Partha (October 2014) Automated placement of stereotactic injections using a laser scan of the skull. (Submitted)

[thumbnail of 1410.5914v1.pdf] PDF
1410.5914v1.pdf - Submitted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (526kB)


Stereotactic targeting is a commonly used technique for performing injections in the brains of mice and other animals. The most common method for targeting stereoscopic injections uses the skull indentations bregma and lambda as reference points and is limited in its precision by factors such as skull curvature and individual variation, as well as an incomplete correspondence between skull landmarks and brain locations. In this software tool, a 3D laser scan of the mouse skull is taken in vitro and registered onto a reference skull using a point cloud matching algorithm, and the parameters of the transformation are used to position a glass pipette to place tracer injections. The software was capable of registering sample skulls with less than 100 micron error, and was able to target an injection in a mouse with error of roughly 500 microns. These results indicate that using skull scan registration has the potential to be widely applicable in automating stereotactic targeting of tracer injections.

Item Type: Paper
Subjects: Investigative techniques and equipment > optical devices > lasers
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Mitra lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 22 October 2014
Date Deposited: 13 Oct 2023 17:30
Last Modified: 13 Oct 2023 17:30
Related URLs:

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving