Parla, J, Kramer, M, McCombie, WR (January 2010) High-Throughput Sequencing. In: Microbial Forensics, Second Edition. UNSPECIFIED, pp. 461-478. ISBN 9780123820068
Abstract
Advances in DNA sequencing methods have a major impact on several biological fields, with microbiology being the first field to experience the benefits of knowing the exact sequence of a genome. This advantage held by the microbiology field was a result of the more compact nature of many microbial genomes, the long-standing history of microbes as model organisms in biological research, and the initial limitations of DNA sequencing chemistry and bioinformatic methods that restricted both the scale and the scope of genomic analysis. The whole genome sequencing of microbial life forms made it feasible to perform functional annotations of genomes and strengthened the motivation of the scientific community to sequence the genomes of several species of medical, environmental, evolutionary, and/or societal importance. With the invention of advanced DNA sequencing methods, exemplified by next-generation sequencers, biological sciences have again been presented with significant opportunities that had not been particularly feasible with more traditional methods. Microbiology has been able to benefit significantly from the functionality of next-generation sequencing. The massive data output of next-generation systems now enables scientists to use whole genome sequencing to identify a microbial isolate rapidly, as well as study microbial sequence variation without having to use various cloning and gene reporter techniques to help isolate the mutation(s) of interest. The independence of the next-generation workflow from microbiological culturing procedures has also promoted the success of metagenomic studies, the results of which have led to the reassessment of long-held scientific views regarding the diversity and interaction within microbial communities.
| Item Type: | Book Section |
|---|---|
| Subjects: | Investigative techniques and equipment > assays |
| CSHL Authors: | |
| Communities: | CSHL labs > McCombie lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | 1 January 2010 |
| Date Deposited: | 29 Sep 2023 18:03 |
| Last Modified: | 29 Sep 2023 18:03 |
| URI: | https://repository.cshl.edu/id/eprint/41074 |
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