Simner, Patricia J, Bergman, Yehudit, Fan, Yunfan, Jacobs, Emily B, Ramakrishnan, Srividya, Lu, Jennifer, Lewis, Shawna, Hanlon, Ann, Tamma, Pranita D, Schatz, Michael C, Timp, Winston, Carroll, Karen C (June 2023) Multicentre genetic diversity study of carbapenem-resistant Enterobacterales: predominance of untypeable pUVA-like blaKPC bearing plasmids. JAC-Antimicrobial Resistance, 5 (3). dlad061. ISSN 2632-1823
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Abstract
OBJECTIVES: Carbapenem-resistant Enterobacterales (CRE) are an urgent public health threat. A better understanding of the molecular epidemiology and transmission dynamics of CRE is necessary to limit their dissemination within healthcare settings. We sought to investigate the mechanisms of resistance and spread of CRE within multiple hospitals in Maryland. METHODS: From 2016 to 2018, all CRE were collected from any specimen source from The Johns Hopkins Medical Institutions. The isolates were further characterized using both phenotypic and genotypic approaches, including short- and/or long-read WGS. RESULTS: From 2016 to 2018, 302 of 40 908 (0.7%) unique Enterobacterales isolates were identified as CRE. Of CRE, 142 (47%) were carbapenemase-producing CRE with KPC (80.3%) predominating among various genera. Significant genetic diversity was identified among all CRE with high-risk clones serving as major drivers of clonal clusters. Further, we found the predominance of pUVA-like plasmids, with a subset harbouring resistance genes to environmental cleaning agents, involved in intergenus dissemination of blaKPC genes. CONCLUSIONS: Our findings provide valuable data to understand the transmission dynamics of all CRE within the greater Maryland region. These data can help guide targeted interventions to limit CRE transmission in healthcare facilities.
| Item Type: | Paper |
|---|---|
| Subjects: | diseases & disorders > Bacterial Infections bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification diseases & disorders bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics diseases & disorders > Bacterial Infections > Antibiotic Resistance bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > plasmid |
| CSHL Authors: | |
| Communities: | CSHL labs > Schatz lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | June 2023 |
| Date Deposited: | 28 Sep 2023 16:05 |
| Last Modified: | 10 Jan 2024 21:13 |
| PMCID: | PMC10214462 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41025 |
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