Gene expression of S100a8/a9 predicts Staphylococcus aureus-induced septic arthritis in mice

Deshmukh, Meghshree, Subhash, Santhilal, Hu, Zhicheng, Mohammad, Majd, Jarneborn, Anders, Pullerits, Rille, Jin, Tao, Kopparapu, Pradeep Kumar (2023) Gene expression of S100a8/a9 predicts Staphylococcus aureus-induced septic arthritis in mice. Frontiers in Microbiology, 14. p. 1146694. ISSN 1664-302X (Public Dataset)

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Abstract

Septic arthritis is the most aggressive joint disease associated with high morbidity and mortality. The interplay of the host immune system with the invading pathogens impacts the pathophysiology of septic arthritis. Early antibiotic treatment is crucial for a better prognosis to save the patients from severe bone damage and later joint dysfunction. To date, there are no specific predictive biomarkers for septic arthritis. Transcriptome sequencing analysis identified S100a8/a9 genes to be highly expressed in septic arthritis compared to non-septic arthritis at the early course of infection in an Staphylococcus aureus septic arthritis mouse model. Importantly, downregulation of S100a8/a9 mRNA expression at the early course of infection was noticed in mice infected with the S. aureus Sortase A/B mutant strain totally lacking arthritogenic capacity compared with the mice infected with parental S. aureus arthritogenic strain. The mice infected intra-articularly with the S. aureus arthritogenic strain significantly increased S100a8/a9 protein expression levels in joints over time. Intriguingly, the synthetic bacterial lipopeptide Pam2CSK4 was more potent than Pam3CSK4 in inducing S100a8/a9 release upon intra-articular injection of these lipopeptides into the mouse knee joints. Such an effect was dependent on the presence of monocytes/macrophages. In conclusion, S100a8/a9 gene expression may serve as a potential biomarker to predict septic arthritis, enabling the development of more effective treatment strategies.

Item Type:	Paper
Subjects:	organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > immunity organism description > animal > mammal organism description > animal > mammal > rodent > mouse organism description > animal > mammal > rodent
CSHL Authors:	Subhash, Santhilal
Communities:	CSHL labs > Beyaz lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	2023
Date Deposited:	21 Sep 2023 18:40
Last Modified:	08 Jan 2024 19:05
PMCID:	PMC10307981
Related URLs:	Publisher
Dataset ID:	GEO: GSE222530
URI:	https://repository.cshl.edu/id/eprint/40952

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