Isolation of mouse mammary carcinoma-derived macrophages and cancer cells for co-culture assays

Sun, Lijuan, Han, Xiao, Egeblad, Mikala (December 2022) Isolation of mouse mammary carcinoma-derived macrophages and cancer cells for co-culture assays. STAR Protocols, 3 (4). p. 101833. ISSN 2666-1667 (In Press)

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Abstract

We recently established an in vitro co-culture system in which monophosphoryl lipid A + interferon-γ (MPLA+IFNγ)-treated tumor-associated macrophages (TAMs) killed cancer cells. Here, we describe a step-by-step protocol for isolating TAMs and cancer cells from mouse primary mammary carcinomas, the setup of the co-culture system, and the image acquisition approach. The technical difficulties in the co-culture assay involve isolating pure TAMs and cancer cells from the same tumor and staining them with different dyes to track the macrophages' tumoricidal activity. For complete details on the use and execution of this protocol, please refer to Sun et al.(2021).

Item Type: Paper
Subjects: diseases & disorders > cancer
Investigative techniques and equipment
organism description > animal
Investigative techniques and equipment > assays
diseases & disorders > cancer > cancer types > breast cancer
Investigative techniques and equipment > cell culture
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > macrophages
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > macrophages
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > macrophages
organism description > animal > mammal
organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions
organism description > animal > mammal > rodent
diseases & disorders > cancer > cancer types
CSHL Authors:
Communities: CSHL labs > Egeblad lab
CSHL Cancer Center Program > Cellular Communication in Cancer Program
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: December 2022
Date Deposited: 22 Nov 2022 18:00
Last Modified: 09 Feb 2024 20:39
PMCID: PMC9664409
URI: https://repository.cshl.edu/id/eprint/40760

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