A-MYB/TCFL5 regulatory architecture ensures the production of pachytene piRNAs in placental mammals

Yu, Tianxiong, Biasini, Adriano, Cecchini, Katharine, Saflund, Martin, Mou, Haiwei, Arif, Amena, Eghbali, Atiyeh, de Rooij, Dirk, Weng, Zhiping, Zamore, Phillip D, Ozata, Deniz M (October 2022) A-MYB/TCFL5 regulatory architecture ensures the production of pachytene piRNAs in placental mammals. RNA. rna.079472.122-rna.079472.122. ISSN 1355-8382

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URL: https://www.ncbi.nlm.nih.gov/pubmed/36241367

DOI: 10.1261/rna.079472.122

Abstract

In male mice, the transcription factor A MYB initiates the transcription of pachytene piRNA genes during meiosis. Here, we report that A MYB activates the transcription factor Tcfl5 produced in pachytene spermatocytes. Subsequently, A MYB and TCFL5 reciprocally reinforce their own transcription to establish a positive feedback circuit that triggers pachytene piRNA production. TCFL5 regulates the expression of genes required for piRNA maturation and promotes transcription of evolutionarily young pachytene piRNA genes, whereas A-MYB activates the transcription of older pachytene piRNA genes. Intriguingly, pachytene piRNAs from TCFL5-dependent young loci initiates the production of piRNAs from A-MYB-dependent older loci ensuring the self-propagation of pachytene piRNAs. A MYB and TCFL5 act via a set of incoherent feedforward loops that drive regulation of gene expression by pachytene piRNAs during spermatogenesis. This regulatory architecture is conserved in rhesus macaque, suggesting that it was present in the last common ancestor of placental mammals.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
CSHL Authors:	Mou, Haiwei
Communities:	CSHL labs > Beyaz lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	14 October 2022
Date Deposited:	20 Oct 2022 02:03
Last Modified:	17 Jan 2024 19:38
PMCID:	PMC9808571
URI:	https://repository.cshl.edu/id/eprint/40735

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