Standard metadata for 3D microscopy

Ropelewski, Alexander J, Rizzo, Megan A, Swedlow, Jason R, Huisken, Jan, Osten, Pavel, Khanjani, Neda, Weiss, Kurt, Bakalov, Vesselina, Engle, Michelle, Gridley, Lauren, Krzyzanowski, Michelle, Madden, Tom, Maiese, Deborah, Mandal, Meisha, Waterfield, Justin, Williams, David, Hamilton, Carol M, Huggins, Wayne (July 2022) Standard metadata for 3D microscopy. Scientific Data, 9 (1). p. 449. ISSN 2052-4463

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Abstract

Recent advances in fluorescence microscopy techniques and tissue clearing, labeling, and staining provide unprecedented opportunities to investigate brain structure and function. These experiments' images make it possible to catalog brain cell types and define their location, morphology, and connectivity in a native context, leading to a better understanding of normal development and disease etiology. Consistent annotation of metadata is needed to provide the context necessary to understand, reuse, and integrate these data. This report describes an effort to establish metadata standards for three-dimensional (3D) microscopy datasets for use by the Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative and the neuroscience research community. These standards were built on existing efforts and developed with input from the brain microscopy community to promote adoption. The resulting 3D Microscopy Metadata Standards (3D-MMS) includes 91 fields organized into seven categories: Contributors, Funders, Publication, Instrument, Dataset, Specimen, and Image. Adoption of these metadata standards will ensure that investigators receive credit for their work, promote data reuse, facilitate downstream analysis of shared data, and encourage collaboration.

Item Type:	Paper
Subjects:	Investigative techniques and equipment organs, tissues, organelles, cell types and functions > organs types and functions > brain Investigative techniques and equipment > microscopy organs, tissues, organelles, cell types and functions > organs types and functions organs, tissues, organelles, cell types and functions
CSHL Authors:	Osten, Pavel
Communities:	CSHL labs > Osten lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	27 July 2022
Date Deposited:	22 Aug 2022 01:26
Last Modified:	17 Jan 2024 14:53
PMCID:	PMC9329339
URI:	https://repository.cshl.edu/id/eprint/40692

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