Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation

Haery, Leila, Deverman, Benjamin, Matho, Katherine, Cetin, Ali, Woodard, Kenton, Cepko, Connie, Guerin, Karen, Rego, Meghan, Ersing, Ina, Bachle, Susanna, Kamens, Joanne, Fan, Melina (September 2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation. BioRxiv. (Unpublished)

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Abstract

Cell-type-specific expression of molecular tools and sensors is critical to construct circuit diagrams and to investigate the activity and function of neurons within the nervous system. Strategies for targeted manipulation include combinations of classical genetic tools such as Cre/loxP and Flp/FRT, use of cis-regulatory elements, targeted knock-in transgenic mice, and gene delivery by AAV and other viral vectors. The combination of these complex technologies with the goal of precise neuronal targeting is a challenge in the lab. This report will discuss the theoretical and practical aspects of combining current technologies and establish best practices for achieving targeted manipulation of specific cell types. Novel applications and tools, as well as areas for development, will be envisioned and discussed.

Item Type:	Paper
Subjects:	organism description > virus > adeno associated virus neurobiology > neuroscience
CSHL Authors:	Matho, Katherine
Communities:	CSHL labs > Huang lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	12 September 2019
Date Deposited:	26 May 2022 17:09
Last Modified:	26 May 2022 17:09
URI:	https://repository.cshl.edu/id/eprint/40638

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