Inducing RNAi in Caenorhabditis elegans by Injection of dsRNA.

Hammell, Christopher M, Hannon, Gregory J (January 2016) Inducing RNAi in Caenorhabditis elegans by Injection of dsRNA. Cold Spring Harbor protocols, 2016 (1). pdb.prot086306. ISSN 1940-3402

URL: https://www.ncbi.nlm.nih.gov/pubmed/26729904
DOI: 10.1101/pdb.prot086306

Abstract

In Caenorhabditis elegans, long double-stranded RNAs (dsRNAs) are overwhelmingly the trigger of choice for inducing RNA interference (RNAi). Although injection of dsRNA into the somatic or germline tissues of animals requires both specific equipment and technical skills, the ability of C. elegans to amplify the initial dsRNA trigger and to transmit the RNAi activity to other somatic tissues and to the progeny of injected animals is one of the main advantages of using C. elegans as a model system. The direct injection of dsRNA into parental animals is the most reliable method for RNAi and also presents the least experiment-to-experiment and animal-to-animal variability.

Item Type: Paper
Subjects: organism description > animal > C elegans
Investigative techniques and equipment > RNAI
CSHL Authors:
Communities: CSHL labs > Hammell C. lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 4 January 2016
Date Deposited: 05 May 2021 13:09
Last Modified: 05 May 2021 13:09
URI: https://repository.cshl.edu/id/eprint/39986

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