Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane

Diniz, A. L., da Silva, D. I. R., Lembke, C. G., Costa, M. D. L., Ten-Caten, F., Li, F., Vilela, R. D., Menossi, M., Ware, D., Endres, L., Souza, G. M. (November 2020) Amino Acid and Carbohydrate Metabolism Are Coordinated to Maintain Energetic Balance during Drought in Sugarcane. Int J Mol Sci, 21 (23). p. 9124. ISSN 1422-0067

URL: https://pubmed.ncbi.nlm.nih.gov/33266228/
DOI: 10.3390/ijms21239124

Abstract

The ability to expand crop plantations without irrigation is a major goal to increase agriculture sustainability. To achieve this end, we need to understand the mechanisms that govern plant growth responses under drought conditions. In this study, we combined physiological, transcriptomic, and genomic data to provide a comprehensive picture of drought and recovery responses in the leaves and roots of sugarcane. Transcriptomic profiling using oligoarrays and RNA-seq identified 2898 (out of 21,902) and 46,062 (out of 373,869) transcripts as differentially expressed, respectively. Co-expression analysis revealed modules enriched in photosynthesis, small molecule metabolism, alpha-amino acid metabolism, trehalose biosynthesis, serine family amino acid metabolism, and carbohydrate transport. Together, our findings reveal that carbohydrate metabolism is coordinated with the degradation of amino acids to provide carbon skeletons to the tricarboxylic acid cycle. This coordination may help to maintain energetic balance during drought stress adaptation, facilitating recovery after the stress is alleviated. Our results shed light on candidate regulatory elements and pave the way to biotechnology strategies towards the development of drought-tolerant sugarcane plants.

Item Type: Paper
Additional Information: 1422-0067 Diniz, Augusto Lima Orcid: 0000-0002-0407-1039 da Silva, Danielle Izilda Rodrigues Lembke, Carolina Gimiliani Costa, Maximiller Dal-Bianco Lamas Ten-Caten, Felipe Orcid: 0000-0001-7771-1490 Li, Forrest Vilela, Romel Duarte Menossi, Marcelo Orcid: 0000-0002-9211-3787 Ware, Doreen Orcid: 0000-0002-8125-3821 Endres, Lauricio Souza, Glaucia Mendes 2014/50921-8/Fundação de Amparo à Pesquisa do Estado de São Paulo/ 2017/02270-6/Fundação de Amparo à Pesquisa do Estado de São Paulo/ 2019/15852-9/Fundação de Amparo à Pesquisa do Estado de São Paulo/ 2013/13659-0/Fundação de Amparo à Pesquisa do Estado de São Paulo/ 2015/01764-0/Fundação de Amparo à Pesquisa do Estado de São Paulo/ 2017/02842-0/Fundação de Amparo à Pesquisa do Estado de São Paulo/ CRIS 8062-2100-044)/U.S. Department of Agriculture/ 304 360/2014-7/Conselho Nacional de Desenvolvimento Científico e Tecnológico/ Journal Article Int J Mol Sci. 2020 Nov 30;21(23):9124. doi: 10.3390/ijms21239124.
Uncontrolled Keywords: Tfbs co-expression network plant stress transcriptome tricarboxylic acid cycle of the study in the collection, analyses, or interpretation of data in the writing of the manuscript, or in the decision to publish the results.
CSHL Authors:
Communities: CSHL labs > Ware lab
Depositing User: Matthew Dunn
Date: 30 November 2020
Date Deposited: 19 Apr 2021 19:58
Last Modified: 19 Apr 2021 19:58
PMCID: PMC7729667
URI: https://repository.cshl.edu/id/eprint/39854

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