Mammary Tumors Induce Central Pro-inflammatory Cytokine Expression, but Not Behavioral Deficits in Balb/C Mice

Walker II, W. H., Borniger, J. C., Surbhi, . , Zalenski, A. A., Muscarella, S. L., Fitzgerald, J. A., Zhang, N., Gaudier-Diaz, M. M., DeVries, A. C. (August 2017) Mammary Tumors Induce Central Pro-inflammatory Cytokine Expression, but Not Behavioral Deficits in Balb/C Mice. Sci Rep, 7 (1). p. 8152. ISSN 2045-2322 (Electronic)2045-2322 (Linking)

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DOI: 10.1038/s41598-017-07596-9


Breast cancer survivors are more likely to develop mood disorders and cognitive deficits than women in the general population. Previous studies suggest that peripheral tumors elicit central pro-inflammatory cytokine production, in turn leading to depression and cognitive deficits. In the current study, two cohorts of female Balb/C mice received bilateral orthotopic injections of syngeneic 67NR, 4T07, or 4T1cells (1 x 10(5) cells per injection) to induce mammary tumors. Approximately three weeks later, learned fear (via fear conditioning) or depressive-like behavior (via tail suspension and forced swim test) was assessed. Proinflammatory cytokine levels were increased in the serum (IL-1beta, TNFalpha, IFNgamma) and livers (IL-1beta, IL-6, TNFalpha) of mice with 4T07 or 4T1 tumors compared to 67NR tumors and the vehicle control. IL-1beta was increased in both the hippocampus and cortex of mice injected with 4T07 or 4T1 cell lines relative to the other treatment groups. However, mammary tumors had no effect on hippocampal doublecortin + and did not alter depressive-like behavior or learned fear. These data demonstrate that similarly sized tumors can produce differential immune responses and that tumor-induced central pro-inflammatory cytokine production can exist in the absence of depressive-like behavior or cognitive deficits.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > organs types and functions > brain
diseases & disorders > cancer > cancer types > breast cancer
diseases & disorders > inflammation > cytokines
diseases & disorders > mental disorders > mood disorders > depression
organism description > animal behavior > fear
CSHL Authors:
Communities: CSHL labs > Borniger lab
Depositing User: Adrian Gomez
Date: 15 August 2017
Date Deposited: 06 Jan 2020 15:06
Last Modified: 06 Jan 2020 15:06
PMCID: PMC5557981
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