Estradiol treatment improves biological rhythms in a preclinical rat model of menopause

Yin, W., Borniger, J. C., Wang, X., Maguire, S. M., Munselle, M. L., Bezner, K. S., Tesfamariam, H. M., Garcia, A. N., Hofmann, H. A., Nelson, R. J., Gore, A. C. (November 2019) Estradiol treatment improves biological rhythms in a preclinical rat model of menopause. Neurobiol Aging, 83. pp. 1-10. ISSN 1558-1497

URL: https://www.ncbi.nlm.nih.gov/pubmed/31585360
DOI: 10.1016/j.neurobiolaging.2019.08.029

Abstract

The perimenopausal transition at middle age is often associated with hot flashes and sleep disruptions, metabolic changes, and other symptoms. Whereas the mechanisms for these processes are incompletely understood, both aging (AG) and a loss of ovarian estrogens play contributing roles. Furthermore, the timing of when estradiol (E) treatment should commence and for how long are key clinical questions in the management of symptoms. Using a rat model of surgical menopause, we determined the effects of regimens of E treatment with differing time at onset and duration of treatment on diurnal rhythms of activity and core temperature and on food intake and body weight. Reproductively mature (MAT, approximately 4 months) or AG ( approximately 11 months) female rats were ovariectomized, implanted intraperitoneally with a telemetry device, and given either a vehicle (V) or E subcutaneous capsule implantation. Rats were remotely recorded for 10 days per month for 3 (MAT) or 6 (AG) months. To ascertain whether delayed onset of treatment affected rhythms, a subset of AG-V rats had their capsules switched to E at the end of 3 months. Another set of AG-E rats had their capsules removed at 3 months to determine whether beneficial effects of E would persist. Overall, activity and temperature mesor, robustness, and amplitude declined with AG. Compared to V treatment, E-treated rats showed (1) better maintenance of body weight and food intake; (2) higher, more consolidated activity and temperature rhythms; and (3) higher activity and temperature robustness and amplitude. In the AG arm of the study, switching treatment from V to E or E to V quickly reversed these patterns. Thus, the presence of E was the dominant factor in determining stability and amplitude of locomotor activity and temperature rhythms. As a whole, the results show benefits of E treatment, even with a delay, on biological rhythms and physiological functions.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > biological clock
bioinformatics > genomics and proteomics > small molecules > estrogen
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat
CSHL Authors:
Communities: CSHL labs > Borniger lab
Depositing User: Adrian Gomez
Date: November 2019
Date Deposited: 06 Jan 2020 14:33
Last Modified: 09 Jan 2020 20:31
PMCID: PMC6858967
Related URLs:
URI: https://repository.cshl.edu/id/eprint/38850

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