Borniger, J. C., Cisse, Y. M., Cantemir-Stone, C. Z., Bolon, B., Nelson, R. J., Marsh, C. B. (May 2016) Behavioral abnormalities in mice lacking mesenchyme-specific Pten. Behav Brain Res, 304. pp. 80-5. ISSN 1872-7549
Abstract
Phosphatase and tensin homolog (Pten) is a negative regulator of cell proliferation and growth. Using a Cre-recombinase approach with Lox sequences flanking the fibroblast-specific protein 1 (Fsp1 aka S100A4; a mesenchymal marker), we probed sites of expression using a beta-galactosidase Rosa26(LoxP) reporter allele; the transgene driving deletion of Pten (exons 4-5) was found throughout the brain parenchyma and pituitary, suggesting that deletion of Pten in Fsp1-positive cells may influence behavior. Because CNS-specific deletion of Pten influences social and anxiety-like behaviors and S100A4 is expressed in astrocytes, we predicted that loss of Pten in Fsp1-expressing cells would result in deficits in social interaction and increased anxiety. We further predicted that environmental enrichment would compensate for genetic deficits in these behaviors. We conducted a battery of behavioral assays on Fsp1-Cre;Pten(LoxP/LoxP) male and female homozygous knockouts (Pten(-/-)) and compared their behavior to Pten(LoxP/LoxP) (Pten(+/+)) conspecifics. Despite extensive physical differences (including reduced hippocampal size) and deficits in sensorimotor function, Pten(-/-) mice behaved remarkably similar to control mice on nearly all behavioral tasks. These results suggest that the social and anxiety-like phenotypes observed in CNS-specific Pten(-/-) mice may depend on neuronal Pten, as lack of Pten in Fsp1-expressing cells of the CNS had little effect on these behaviors.
| Item Type: | Paper |
|---|---|
| Subjects: | diseases & disorders > mental disorders > anxiety disorders bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > PTEN organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus organism description > animal > mammal > rodent > mouse |
| CSHL Authors: | |
| Communities: | CSHL labs > Borniger lab |
| Depositing User: | Adrian Gomez |
| Date: | 1 May 2016 |
| Date Deposited: | 03 Jan 2020 19:17 |
| Last Modified: | 03 Jan 2020 19:17 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/38843 |
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