Active drift stabilization in three dimensions via image cross-correlation

Koo, P. K., Setru, S. U., Mochrie, S. G. (October 2013) Active drift stabilization in three dimensions via image cross-correlation. Rev Sci Instrum, 84 (10). p. 103705. ISSN 0034-6748

DOI: 10.1063/1.4824197


By monitoring stage drift via the normalized cross-correlation of an image of a stuck bead, obtained in real-time, with an out-of-focus "template" image of a similar immobile bead, stored in memory, we implement a simple approach to actively stabilize drift in all three dimensions for existing video microscopy setups. We demonstrate stability to 0.0062 nm along the Z-axis and 0.0031 nm along the X- and Y-axes for long (100 s) timescales.

Item Type: Paper
Subjects: Investigative techniques and equipment > microspheres
Investigative techniques and equipment > imaging
CSHL Authors:
Communities: CSHL labs > Koo Lab
Depositing User: Matthew Dunn
Date: October 2013
Date Deposited: 16 Sep 2019 16:23
Last Modified: 16 Sep 2019 16:27
Related URLs:

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving