Active drift stabilization in three dimensions via image cross-correlation

Koo, P. K., Setru, S. U., Mochrie, S. G. (October 2013) Active drift stabilization in three dimensions via image cross-correlation. Rev Sci Instrum, 84 (10). p. 103705. ISSN 0034-6748

URL: https://www.ncbi.nlm.nih.gov/pubmed/24182117

DOI: 10.1063/1.4824197

Abstract

By monitoring stage drift via the normalized cross-correlation of an image of a stuck bead, obtained in real-time, with an out-of-focus "template" image of a similar immobile bead, stored in memory, we implement a simple approach to actively stabilize drift in all three dimensions for existing video microscopy setups. We demonstrate stability to 0.0062 nm along the Z-axis and 0.0031 nm along the X- and Y-axes for long (100 s) timescales.

Item Type:	Paper
Subjects:	Investigative techniques and equipment > microspheres Investigative techniques and equipment > imaging
CSHL Authors:	Koo, Peter K
Communities:	CSHL labs > Koo Lab
Depositing User:	Matthew Dunn
Date:	October 2013
Date Deposited:	16 Sep 2019 16:23
Last Modified:	16 Sep 2019 16:27
Related URLs:	Publisher http://repository.cshl.edu/38388/
URI:	https://repository.cshl.edu/id/eprint/38389

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