Abdeladim, L., Matho, K. S., Clavreul, S., Mahou, P., Sintes, J. M., Solinas, X., Arganda-Carreras, I., Turney, S. G., Lichtman, J. W., Chessel, A., Bemelmans, A. P., Loulier, K., Supatto, W., Livet, J., Beaurepaire, E. (April 2019) Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy. Nat Commun, 10 (1). p. 1662. ISSN 2041-1723
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Abstract
Large-scale microscopy approaches are transforming brain imaging, but currently lack efficient multicolor contrast modalities. We introduce chromatic multiphoton serial (ChroMS) microscopy, a method integrating one-shot multicolor multiphoton excitation through wavelength mixing and serial block-face image acquisition. This approach provides organ-scale micrometric imaging of spectrally distinct fluorescent proteins and label-free nonlinear signals with constant micrometer-scale resolution and sub-micron channel registration over the entire imaged volume. We demonstrate tridimensional (3D) multicolor imaging over several cubic millimeters as well as brain-wide serial 2D multichannel imaging. We illustrate the strengths of this method through color-based 3D analysis of astrocyte morphology and contacts in the mouse cerebral cortex, tracing of individual pyramidal neurons within densely Brainbow-labeled tissue, and multiplexed whole-brain mapping of axonal projections labeled with spectrally distinct tracers. ChroMS will be an asset for multiscale and system-level studies in neuroscience and beyond.
Item Type: | Paper |
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Subjects: | Investigative techniques and equipment > imaging Investigative techniques and equipment > optical devices > microscope techniques or equipment |
CSHL Authors: | |
Communities: | CSHL labs > Huang lab |
Depositing User: | Matthew Dunn |
Date: | 10 April 2019 |
Date Deposited: | 16 Apr 2019 15:08 |
Last Modified: | 16 Apr 2019 15:08 |
PMCID: | PMC6458155 |
Related URLs: | |
URI: | https://repository.cshl.edu/id/eprint/37781 |
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