Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy

Abdeladim, L., Matho, K. S., Clavreul, S., Mahou, P., Sintes, J. M., Solinas, X., Arganda-Carreras, I., Turney, S. G., Lichtman, J. W., Chessel, A., Bemelmans, A. P., Loulier, K., Supatto, W., Livet, J., Beaurepaire, E. (April 2019) Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy. Nat Commun, 10 (1). p. 1662. ISSN 2041-1723

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URL: https://www.ncbi.nlm.nih.gov/pubmed/30971684

DOI: 10.1038/s41467-019-09552-9

Abstract

Large-scale microscopy approaches are transforming brain imaging, but currently lack efficient multicolor contrast modalities. We introduce chromatic multiphoton serial (ChroMS) microscopy, a method integrating one-shot multicolor multiphoton excitation through wavelength mixing and serial block-face image acquisition. This approach provides organ-scale micrometric imaging of spectrally distinct fluorescent proteins and label-free nonlinear signals with constant micrometer-scale resolution and sub-micron channel registration over the entire imaged volume. We demonstrate tridimensional (3D) multicolor imaging over several cubic millimeters as well as brain-wide serial 2D multichannel imaging. We illustrate the strengths of this method through color-based 3D analysis of astrocyte morphology and contacts in the mouse cerebral cortex, tracing of individual pyramidal neurons within densely Brainbow-labeled tissue, and multiplexed whole-brain mapping of axonal projections labeled with spectrally distinct tracers. ChroMS will be an asset for multiscale and system-level studies in neuroscience and beyond.

Item Type:	Paper
Subjects:	Investigative techniques and equipment organism description > animal organs, tissues, organelles, cell types and functions > cell types and functions > cell types > astrocytes organs, tissues, organelles, cell types and functions > cell types and functions > cell types > astrocytes organs, tissues, organelles, cell types and functions > cell types and functions > cell types > astrocytes organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex Investigative techniques and equipment > microscopy > flourescence microscopy Investigative techniques and equipment > imaging organism description > animal > mammal Investigative techniques and equipment > microscopy Investigative techniques and equipment > optical devices > microscope techniques or equipment organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions organism description > animal > mammal > rodent organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:	Matho, Katherine
Communities:	CSHL labs > Huang lab
Depositing User:	Matthew Dunn
Date:	10 April 2019
Date Deposited:	16 Apr 2019 15:08
Last Modified:	01 Feb 2024 20:01
PMCID:	PMC6458155
Related URLs:	https://www.nature.com/articles/s41467-0...
URI:	https://repository.cshl.edu/id/eprint/37781

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